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Evidence has been accumulating that calpains are necessary to initiate the degradation of myo brillar proteins by releasing them from the surface of the myo bril and making them available for subsequent degradation. Given that calpains cleave proteins at a limited number of sites and produce large polypeptide fragments rather than small peptides or amino acids, it is clear that other proteases may be involved in the bulk degradation of sarcomeric structures (Goll et al. 2003). For the subsequent breakdown of myo brillar proteins, once calpains have released them from the sarcomere, the main candidate is the proteasome (Attaix et al. 1998, 2001; Delbarre-Ladrat et al. 2006; Yamamoto et al. 2009; Geesink and Veiseth 2009). The proteasome, or multicatalytic proteinase complex (MCP), is a multisubunit protease complex with an apparent sedimentation coef cient of 20S. Two types of regulatory complexes bind to both ends of the cylindrical 20S. One complex, the 26S proteasome, is a eukaryotic ATP-dependent protease (Tanaka 1998) and hydrolyzes ubiquitinconjugated proteins (Tanaka 1998; Delbarre-

kava, 600 research summary, 601 St. Johns wort (SJW), 600 601 integrating psychological interventions with, 607 mind/body therapies, 606 movement therapies, 604 605 aerobic exercise, 604 Alexander technique (movement therapy), 605 Feldenkrais functional integration (movement therapy), 605 leisure activity, 604 resistance exercise, 604 yoga, 605 tactile therapies, 602 605 aromatherapy, 603 energy mobilization, 603 massage, 603 myofascial release and Rol ng, 603 polarity therapy, 604 re exology, 603 reiki, 604 soft tissue therapies, 603 therapeutic touch (TT), 603 604 Western systems of healing (therapeutic approaches/clinical applications), 592 595 chiropractic, 594 595 homeopathy, 592 593 osteopathy, 593 594 Tobacco use/dependence, 147 162 advertising/promotions and, 154 aging and, 492, 494, 497 animal models of nicotine addiction, 149 153 place-conditioning (PC), 150, 151 152 preclinical genetic models: insights into individual differences, 152 relevance of preclinical studies to understanding tobacco dependence, 152 153 self-administration, 149 151 validity of, 150 basic mechanisms of nicotine addiction, 148 149 cancer and, 270 cessation methods (pharmaceutical/ nonpharmaceutical), 155 156, 596 cognitive effects, 153 ethnic minorities: African Americans, 546 Asian Americans, 547 548 differences, 153 interventions for, 551 552 Latino(a) Americans, 549 Native Americans, 550 gender differences, 153 genetic in uence evidence, 157 160 instruments for measuring, 159 160 phenotypes, 158 159 prevention/treatment, 155 157 pricing/control policy effects, 154 155 psychiatric comorbidity, 153 154 public health imperative, 160 161 risk factors for initiation/maintenance, 153 155 tobacco dependence construct in need of re nement, 159 160

For chi-square and multiway frequency analysis, examine expected cell frequencies for all two-way relationships to ensure that all are greater than 1 and that no more than 20% are less than 5 Except in some applications of chi-square, inadequate expected frequencies do not lead to increased Type I error but rather to reduction in power, which can become notable as expected frequencies for two-way associations drop below 5 in some cells (Milligan, 1980) If low expected frequencies are encountered, several choices are available First, you can simply choose to accept reduced power Second, you can collapse categories for variables with more than two levels For example, you could collapse the categories three and four or more into one category of three or more The categories you collapse should be carefully considered because it is quite possible that associations will disappear as a result.

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Because this is equivalent to a complete reduction in power for testing those associations, nothing has been gained Finally, you can delete variables to reduce the number of cells as long as care is taken to delete only variables that are not associated with the remaining variables For example, in a table with three discrete variables you might consider deleting a variable if there is no three-way association and if at least one of the two-way associations with the variable is nonsigni cant (Milligan, 1980) The common practice of adding a constant to each cell is not recommended because it has the effect of further reducing power Its purpose is to stabilize Type I error rate, but as noted earlier, that is generally not the problem, and other remedies are available when it is.